?????? - TEAC

???????????????????????. ???????????????????????????????????????????.







? ????????????????
??????????????????????????????. ????? CDMO ???????????? ???????????????????????? ...
?????????? ???1?
?????????????????????????TD???. ???? ... ?????????????????????????????????????? ...
?CD40 ligand ??????????????????HepG2 ?? ...
???????????????????????PAC????pac???????????????????????. ????????????????????????? ...
TRAF6 shRNA?LPS/TLR4?????????????
?????. ??????????EpllBZFN???????. ??????????????????I:N. ???????????????????GV287?E?B2.
smad4 shRNA ???????????? - ??????
?????????????????,???????. ??????????????????????????. ???????????????[1]?
(12)??????
... ???????pMD19-T simple ???????????????? F17-G ?? ... pMAL-c4x ??????????????BL21 ???????????????? ...
???????????????? - CORE
????????pEGFP-C2-NLRC5 ????????. ??RAW264? 7 ????????????????. RAW264? 7 ???NLRC5 ???cDNA??EcoR ? ?.
?????????????????pMD?pac??
??. PCR?????DNA???????, pET- 28a( + ) - A rp2/3????????? ... PVDF?(??M illipore??); ???????(?????. ???????); ...
????????????
?2 ?????????????. Fig. 2 The construction map of full2length genomic chimeric clones. ??????????????????????. ?????? ...
??pEGFP-C2-NLRC5 ??????????? - ????????
??????TaKaRa?Sangon ???? 1.1.4 ?????TD20/20 ?????(??Promege ??)? 1.2 ??. 1.2.1 ??c-myc ????????pcDNA3.1/c-myc?5%CO2 ...
??????????????????? - ??CPH
DNA?Sigma?????????????Sal??Mlu??. Hind??Nhe??Pst? ... ????????????????????. ??CIp10[3]???? 1.3.1 ?? ...
?????EIAV?????????????????????
?????????????DNA Ligation Kit Ver.2.1?????(Code No ... ??????3?????FN-TD-1??????SDS-PAGE???? ...