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?CD40 ligand ??????????????????HepG2 ?? ...???????????????????????PAC????pac???????????????????????. ????????????????????????? ... TRAF6 shRNA?LPS/TLR4??????????????????. ??????????EpllBZFN???????. ??????????????????I:N. ???????????????????GV287?E?B2. smad4 shRNA ???????????? - ???????????????????????,???????. ??????????????????????????. ???????????????[1]? (12)??????... ???????pMD19-T simple ???????????????? F17-G ?? ... pMAL-c4x ??????????????BL21 ???????????????? ... ???????????????? - CORE????????pEGFP-C2-NLRC5 ????????. ??RAW264? 7 ????????????????. RAW264? 7 ???NLRC5 ???cDNA??EcoR ? ?. ?????????????????pMD?pac????. PCR?????DNA???????, pET- 28a( + ) - A rp2/3????????? ... PVDF?(??M illipore??); ???????(?????. ???????); ... ?????????????2 ?????????????. Fig. 2 The construction map of full2length genomic chimeric clones. ??????????????????????. ?????? ... ??pEGFP-C2-NLRC5 ??????????? - ??????????????TaKaRa?Sangon ???? 1.1.4 ?????TD20/20 ?????(??Promege ??)? 1.2 ??. 1.2.1 ??c-myc ????????pcDNA3.1/c-myc?5%CO2 ... ??????????????????? - ??CPHDNA?Sigma?????????????Sal??Mlu??. Hind??Nhe??Pst? ... ????????????????????. ??CIp10[3]???? 1.3.1 ?? ... ?????EIAV??????????????????????????????????DNA Ligation Kit Ver.2.1?????(Code No ... ??????3?????FN-TD-1??????SDS-PAGE???? ... ?18????????????????????. 201. ???. ????. V TD TR. 1. ?? ??. ??FC. 1 v. 5. 1. 6. 8. 2. ?? ?. ??JFC v. 5 v. 5. 2 10 2. 3. ?? ??. ??FC. 0. 0. 0. ?????. D [û×üë? 2024(.xlsm???????????2019 ?. ?????????168 ?????. ?2020 ???142 ????????. ????????????????.
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