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International Center Courses - Keio University
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Clonal heterogeneity of endocrine therapy resistance in breast cancer
2.2.1.4 Thawing Cells ... Chemotherapy is the use of drugs or drug combinations that are capable of killing tumor cells as a treatment method.
Doxorubicin and ?-Mangostin oppositely affect luminal breast ...
cell lines more usually the number of subcultures since last thawed from storage. ... claimed tissues of origin are in fact MCF-7 (breast cancer) or T24. (bladder ...
A Model to Study Metastatic Cell Adhesion and Anti-Cancer Drugs
A fixed number of MCF-7 breast cancer cell line cells were subjected to fixation with ... For both fresh cells and DSP fixed cells, cryopreservation and thawing ...
reversal of paclitaxel resistance in mcf-7 cell line by a chemical ...
MDA?MB?231 and MCF?7 cells were transfected with Lipofectamine® 3000 (Invitrogen;. Thermo Fisher Scientific, Inc.) according to the manufacturer's protocols.
Apoptotic effects of human amniotic fluid mesenchymal stem cells ...
Remark: Cell viability is dependent on the rapid and complete thawing of frozen cells. ... A protocol was developed for the expansion of MCF-7 ...
Imbalance of Antioxidant Enzymes in Tumor Cells and Inhibition of ...
The aim of this guidance document is to reduce the uncertainties in cell and tissue-based in vitro method derived predictions by applying all necessary good ...
reversal of multidrug resistance in mcf-7 breast adenocarcinoma
MCF-7 wild-type cells (MCF-7/WT) were transfected with. Fugene #6 lipofecting agent, according to the manufac- turers protocol. Cells (2 105) were cotransfected ...
Guidelines for the use of cell lines in biomedical research
We analyzed the impact of each method on the cell adsorption rate onto ConA beads using MCF-7 cells. Based on these optimization experiments ...
Optimising single-cell gene expression profiling of circulating tumour ...
Using this method, we tested the change of the cell properties of human cancer breast epithelial cell line, MCF-7 ... After thawing initially frozen cells ...
D3.3 Report on characterization in vitro human/animal mammary ...
An aliquot of soluble protein was obtained by one cycle of freezing/thawing the cells, vortexing (30 sec), and centrifuging at 12,000 ? g (1 min) ...