Guidelines for the use of cell lines in biomedical research
We analyzed the impact of each method on the cell adsorption rate onto ConA beads using MCF-7 cells. Based on these optimization experiments ...
Optimising single-cell gene expression profiling of circulating tumour ...Using this method, we tested the change of the cell properties of human cancer breast epithelial cell line, MCF-7 ... After thawing initially frozen cells ... D3.3 Report on characterization in vitro human/animal mammary ...An aliquot of soluble protein was obtained by one cycle of freezing/thawing the cells, vortexing (30 sec), and centrifuging at 12,000 ? g (1 min) ... Guidance Document on Good In Vitro Method Practices (GIVIMP)Total cell homoge- nate was centrifuged at 15,000 3 g for 10 min, and the supernatant was collected and used for enzyme activity assay or immunoblot analysis. EpiCypher® CUTANA? Direct-to-PCR CUT&Tag ProtocolNanomaterials applied to medicine have been showing great potential and offer innovative strategies to the diagnosis, prevention, and treatment of several. University of Groningen Nanobiomaterials for biological barrier ...Higher levels of EGFR were detected in. TNBC cells compared to the non-TNBC cell line MCF-7, which does not express EGFR. Levels of ... Anti-EGFR monoclonal antibodies and EGFR tyrosine kinase ...2.2.1.4 Thawing Frozen Cells. Because of the toxic effect DMSO which above 4 0C to the cells, thawing the frozen cells quickly is very important at 37 0C ... METASTATIC BEHAVIOUR OF DOXORUBICIN RESISTANT MCF-7 ...Both estrogen-independent and tamoxifen-resistant derivatives of MCF-7 have been selected using a protocol that mimics disease progression in patients. ( ... ?????????? - UNCTAD???????????????????????????????. ??????????????????????????????. ???????????????? ... Untitled - googleapis.com????(????),?????????. ???????????????????. ???????????????????. ??????????????????? ? ... ???????? - GEF?????????????????????????, ???, ????, ??. ??, ???, ??, ????, ???, ??, ??, ???????, ????, ??. ????????. ? ??????????. ?????????????? ... ???????????????SMQ ????. ???????????? ????? ... ??????????????. ??????????????????????????2011 ?7 ?14. ????????????????????
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