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??pTarget(??)?????5??,????????1???2? ?????pTarget ??,pTarget(??)?????Gibson ??,pTarget (??)?????. ??Gibson ...

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Untitled - Googleapis.com

(1) ??pTarget-jiV??:?pTarget F??(Addgene Plasmid #62226). ???,?pT-yyjiV-F/R?????PCR??,PCR????????????,. ??Dpn I ????37 ...

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