P250 - türk biyokimya dergisi
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oordinator
0.Hamdi Ö ü_, Co-Coordinator
Nurhan Pural1, Secretary
N.Leyla Açan
Nejat Dalay
Aleksandar Dimovski
Burak Erman
Ömer Güzel
Mustafa Gültepe
Vas1f N.Has1rc1
Ivan Ivanov
Dusanka Janazic
Beki Kan
Güldal K1rkali
Eugenia Kovacs
Zdravko Lalchev
Yahya Laleli
Ay Ö ü_
H.Avni Öktem
Cihan Öner
0nci Özer
Asuman Özkara
Rüstem Nurten
Erhan Pi_kin
Zehra Sayers
Vesna Svetlicic
Yusuf Tan
Tijen Tanyalç1n
Azmi Telefoncu
Asl1han Tolun
Belma Turan
Engin Ulukaya
Figen Zihnio lu
Matjas Zorko
Advisory Board K1ymet Aksoy
T.Aslan Aksu
Emel Ar1nç
Diler Aslan
Erol Atalay
Sevil Atasoy
U ur Atik
Ebubekir Bakan
Oya Bay1nd1r
Cumhur Bilgi
Bora Barutçu
Kutlay Burat
Gülden Burçak
Naime Canoruç
Gönenç Ciliv
Salih Çelik
Ömer Çolak
I_1l Çokur
0lhami Demirel
Mübeccel Durusoy
A.Kaya Emerk
Ahmet Eraslan
Füsun Erciyes
Nurten Erdal
Biltan Ersöz
Hamza Esen
Sina Gökçe
^endo an Gülen
0smail Gülen
Gül Üner
Münire Hac1bekiro lu
Levent Karaca
Baysal Karaca
Hilal Karagül
Levent Kayr1n
Edip Keha
Nedret K1l1nç
Yüksel Koca
^ebnem Kösebalaban
Mehmet Köseo lu
Yusuf Kurtulmu_
Türker Kutluay
Sevnur Mandalc1
Taner Onat
Asuman Orçun
Sinan Önen
Tomris Özben
Meral Özgüç
Ferit Pehlivan
Gül Saydam
Ahmet Sivas
Zerrin Söylemez
Bolkan ^im_ek
Yavuz Taga
E.Ferhan Tezcan
Demir Tiryaki
Asuman Tokullugil
I_1k Türkalp
Suna Türko lu
Müjdat Uysal
Ali Y1lmaz
Piraye Yarg1ço lu
Meral Yüce
Do an Yücel
Güne_ Yüre ir
SCIENTIFIC PROGRAM FOR BIOCHEMICAL & BIOPHYSICAL DAYS
OCTOBER 12, 2003 SUNDAY09:00 - 17:00RegistrationHall C17:00Opening Ceremony17:30Opening LectureChair: Nazmi ÖzerNovel approaches to genetic diseases
P1nar T. Özand18:30Break20:00Welcome Cocktail
OCTOBER 13, 2003 MONDAYHall AHall BChair: George Russev, Mesude 0_canChair: Stefan Szedlacsek, Beki Kan09:00Molecular Basis of Colorectal Cancer in The Republic of Macedonia
Alexander DimovskiExploring The Interaction of Seven-Transmembrane Receptors With G-Proteins By Using Synthetic Model Peptides
Matjaz Zorko09:40Irregular Ionisation of Proteins. An Alternative Interpretation of Experimental Observations
Andrey KarshikoffOncogenic Signalling Pathways and Cell Death Based Tumour Therapies
Alexander Pintzas10:20From Microparticles to Giant Cells of Northern Adriatic
Vesna SwetlicicModelling of Cellular Receptor Signalling Pathways
Haluk Re_at11:00Coffee BreakChair: Vladimir Mrsa, Figen Zihnio luChair: Dusanka Janezic, Kevser Pi_kin11:30A Simple Spectro photometric Method for the determination of Selenium in Biological Materials
Burcu OkutucuHuman Interferon Gamma-Significance of the C-Terminal Flexible Domain for its Biological Activity
Genoveva Nacheva11:50FT-IR Spectroscopic Studies of Melatonin - Induced Changes in Rat Liver Microsomes
Güvenç GörgülüNon-Enzymatic Glycosylation of Recombinant Human Interferon-Gama
Roumyana Mironova12:10Molecular Investigation of The Effect of Melatonin on Rat Brain Tissue
Sara Banu Akka_Noninvasive Optical Assay for Synthetic Hemoglobin Fluids
Mehmet Dinçer Bilgin12:30A Rat Dementia Model by Chronic Ethanol Consumption and Withdrawal: Validation by Passive Avoidance Measurement and Serum Cholinestrase Level
Erdinç Çak1rThe Status of Patients with Diabetes Mellitus Monitored by Turkish Diabetes Society in Denizli / Turkey
Diler Aslan13:00Lunch14:00Poster17:00Free Time
OCTOBER 14, 2003 TUESDAYHall AHall BChair: Alexander Dimovski, Zoe LygerouChair: Andrey Karshikoff, Gürbüz Çelebi09:00Global Genomic and Transcription-Coupled DNA Repair Rates in Human Cells
George RussevYeast Cell Wall Proteins: Localisation, Function, Application
Vladimir Mrsa09:40Cloning, Expression and Preliminary Characterization of Xylulose-5-Phosphate Phospoketolase From Lactococcus lactic.
Stefan SzedlacsekCatabolic and Anabolic Agents of Bone Organ - Culture
Emina Sofic10:20Y-Chromosome Microdeletions Among Infertile Men From The Republic of Macedonia
Dijana Plaseska-KaranfilskaElectro-manipulation of The Biological Cells: Basics and Applications
Mihai Radu11:00Coffee BreakChair: Ivan Ivanov, Dijana Plaseska-Karanfilska, 0. Hamdi Ö ü_Chair: Ivan Ivanov, Azmi Telefoncu11:30Screening of RPGR Gene in Turkish Retinitis Pigmentosa Patients
Ceren AcarImmunoliposomes Directed Toward VCAM-1 : Vehicles for Specific Drug Delivery to Activated Endothelial Cells
Manuela Vionea11:50Methylation Pattern and Dnasel Hypersensitivity within the Intregenic Spacer of Ribosomal RNA Genes in Excised Cotyledons of Cucurbita Pepo L. ( Zucchini ) after Cytokinin Treatment
Evgeni AnanievDevelopment of two Amperometric Biosensors Based on Catalase Immobilized in Gelatin - Alignate and Gelatin-k-Carrageenan for Alcohol Determination
Erol Aky1lmaz12:10Gene Polymorphism of Endothelial Markers in Renal Dysfunction
Constantina HeltianuDetermination of Chromium ( VI ) by a Catalytic Spectrophotometric Method in the Presence of p-Aminobenzoic Acid
Angelina Stoyanova12:30Expression of Estrogen in Breast Carcinomas
Vesna Vranic MandusicAltered Drug Resistance and Neurologic Disorders in Drosophila Melanogaster with a Deficient Histamine-Gated
Chloride Channel
Eugene Semenov13:00Lunch14:00Tour To Ephesus
OCTOBER 15, 2003 WEDNESDAYHall AHall BChair: Matjaz Zorko, Alexander PintzasChair: Vesna Swetlicic, Mihai Radu09:40The Roles of CYP1A and CYP2E in Chemical Carcinogenesis
Emel Ar1nçInactivation of Melanoma Cells Irradiated with Gamma-Rays and Low Energy Protons
Alexandra Ristic-Fira10:20Cell Cycle Regulation and Genome Integrity
Zoe LygerouLarge Scale Macromolecular Simulations By Simplectic Integration Methods
Dusanka Janezic11:00Coffee Break Chair: Alexandra Ristic-Fira, Leyla AçanChair: Necla Öztürk, Vassilj Goltsev11:30The Effect of Insulin Level on Steroid Receptors
Mojka VulovicImplementation of a Neovascular Coupling Model
Ata Ak1n11:50Molecular Analysis of the ABCA4 Gene in Turkish Patients with Stargardt Disease and Retinitis Pigmentosa
R1za Köksal ÖzgülAnalysis of Millisecond Delayed Fluorerescene Dark Decay
Vassilj Goltsev12:10Mitochondrial Enzyme Activities and mtDNA Polymorphism in Parkinson's Disease
Gülnihal Kulaks1zDevelopment of an Analogue Model Simulating the Portal Vein
Necla Öztürk12:30Determination of Steadt-State Levels of OxoGuanine in Calf Thymus DNA by Means of FPG Protein
Beran Yoku_Purification and Characterization of A PSTS Hyperalkaline Phosphates from T.Thermophilus
Anastasia Pantazaki13:00Lunch14:00PosterClosing Ceremony17:30Free Time20:00Farewell Dinner
SCIENTIFIC PROGRAM FOR MEETING ON METABOLIC DISORDERS
OCTOBER 13 , 2003 MONDAYHall CChairmen: A. Kaya Emerk, I_1k Türkalp09:00Ortado uda Genel Problem Olan Homosistinürinin üç Tipi Three Types of Homocystinuria A Common Problem in Middle East
P1nar T. Özand10:05Yenido anda Konjenital Hipotiroidi Taramas1 Screening for Congenital Hypothyroidism in theNeonate
Alev Özön10:20Yenido anda Tandem-Mass Spektrometre ile Geni_ Kapsaml1 Kal1tsal Metabolik Hastal1klar1n Taranmas1 Expanded Newborn Screening for inborn Errors of Metabolism by Tandem Mass Spectrometry
0nci Karaaslan10:40Tart1_ma/ Discussion11:00Coffee BreakChairmen: Fatma Z. Kutay, Nezih Hekim11:30Kitle Tarama Testlerinin Uygulan1_1nda Kalite Kontrolunun Önemi ve Kitle Tarama Verilerinde Referans Aral1 1n1n Saptanmas1 The Use of Split-Sample Design for Performance Evaluation of Screening Kits: A Real Life Study
Tijen Tanyalç1n11:50Fetusta Biyokimyasal Tan1 Foetal Biochemistry: Biochemical Diagnosis for Foetus
Nezih Hekim12:10Tart1_ma / Discussion13:00Lunch14:00 - 16:30Poster17:00 - 19:00Panel : Türkiye'de Neonatal Tarama Politikas1 Neonatal Screening Policy of Turkey
Chair: U ur Dündar / Mine Özbek
P1nar T. Özand, 0mran Özalp, Münip Üstünda , Yahya Laleli, Mine Özbek
OCTOBER 14 , 2003 TUESDAYHall CChairmen: Yahya Laleli, Tijen Tanyalç1n09:45Amino Asit Metabolizmas1 Bozuklu unda Kullan1lan Metodlar ve Kar_1la_1lan Güçlükler Amino Acid Analysis Methods-Details and Difficulties
Gürsel Bibero lu10:15The Use of Tandem Mass Spectrometry in Newborn Mass Screening Programs: The Facts Beyond the Myth
François Eyskens11:00Coffee BreakChairmen: Aslan Aksu, Sema T. Ozan11:30Karbohidrat Metabolizmas1 Hastal1klar1: Klinik Yakla_1m, Te_his ve Tedavide Laboratuvardan Beklentiler Disorders of Carbohydrate Metabolism : Clinical Approach Future Prospects of Diagnosis and Treatment
Benal Büyükgebiz12:00Karbohidrat Metabolizmas1 Hastal1klar1: Metodlar ve Kar_1la_1lan Güçlükler Disorders of Carbohydrate Metabolism Basic Concepts, Evaluation of Laboratory Methods and Difficulties Observed
Aslan Aksu12:30Tart1_ma / Discussion13:00Lunch14:00Tour to Ephesus
OCTOBER 15, 2003 WEDNESDAYHall CChairmen: Güldal K1rkal1, Hakan Ayd1n09:00Obesite, Polikistik Over ve 0nsülin Direnci Dsyregulation of P450C17 Enzyme in Polycystic Ovary Syndrome
Fahrettin Kele_timur09:30Obesite: Nöral Mekanizmalar Neuroendocrine System and Obesity
Üstün Korugan10:00Obesite Bir 0nflamasyon mudur? Is Obesity an Inflammatory Disease ?
Cande er Y1lmaz10:30Tart1_ma / Discussion11:00Coffee BreakChairmen: Orhan De er, Nezaket Eren11:30Hiperlipoproteinemiler ve Di er Lipid Metabolizmas1 Hastal1klar Laboratory Diagnosis in Lipoprotein and Other Lipid Metabolism Disorders
Gülçin Eskandari12:00Glikosfingolipidozlar: Hastal1ktan Metabolizman1n Temel 0lkelerine The Glycosphingolipidoses: From Disease to Basic Principles of Metabolism
Asuman Özkara12:30Tart1_ma / Discussion13:00Lunch14:00Poster17:00Closing17:30Free Time20:00Fairwell Party
OCTOBER 12, 2003 - SUNDAYHALL C
OPENING LECTURE
NOVEL APPROACHES TO GENETIC DISEASES
Pinar T. Ozand MD,Ph. D., Chairman,
Department of Genetics, King Faisa Specialist Hospital & Research Centre. PO Box 3354, Riyadh 11211, Saudi Arabia.
The objective of this presentation is to present the novel strategies adopted to prevent genetic diseases in Saudi Arabia. The genetic diseases either due to chromosome defects or to alterations in single gene structure produce infants with usually severe diseases.
The management of such disorders is: (A) usually costly due to the fact the morbidity lasts for many years, (B) not always successful and doesnt always produce good results and (C) disrupts the normal family function. These diseases, particularly those inherited single gene disorders plague the communities with consanguineous marriages. Based on our experience in the Kingdom, we have adopted several strategies to combat this public health problem. The methods to combat genetic diseases include: (1) if nothing else is possible to perform a neonatal screening program for treatable diseases. This must be done within 2-3 days after birth. (2) Preimplantation diagnosis. Initially we have focused on six major single gene diseases of the country: MSUD, biopterin dependent PKU, homocystinuria, propionic acidemia, Niemann Pick disease type B and Gaucher disease type A. All of these diseases are either difficult to manage requiring the devotion of many clinical hours by the physician or their procedures of management or are extremely costly. (3) Premarital screening. Applying the mutations on a DNA chip and screening the extended family as well as population for carriers of these diseases. (4) Chromosome abnormalities to be studied by CGS (complete human genome screening) that can be applied to a newborn as well as in preimplantation efforts. This presentation will detail these approaches and our preliminary results.
The result with preimplantation intervention in an ataxia-telangiectasia family will be presented.
OCTOBER 13 , 2003 MONDAYHALL A
LECTURE 1
MOLECULAR BASIS OF COLORECTAL CANCER IN THE REPUBLIC OF MACEDONIA
Aleksandar J DIMOVSKI1,2, Dijana Plaseska-Karanfilska 1), Ana-Marija STEFANOVSKA1, Georgi D.EFREMOV 1)
1) Macedonian Academy of Sciences and Arts, Research Center for Genetic Engineering and Biotechnology 2) Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Skopje, Republic of Macedonia
Correspondence: adimovski@baba.ff.ukim.edu.mk
Colorectal cancer (CRC) is one of the most common cancers and the second cause of death in developed countries. In addition to environmental factors, genetic predisposition has a significant role in the ethiopathogenesis of the disease. Apart from the two dominantly inherited syndromes (FAP and HNPCC) several low penetrance genes were implicated in the initiation of colorectal cancerogenesis. The aim of this study was to determine the molecular basis of FAP, the incidence of HNPCC and the frequency of polymorphisms in several low penetrance genes (I1307K and E1317Q in the APC gene, TbðRI(6A) and CCND1) associated with CRC. A total of 173 patients with CRC, of which six patients with multiple adenomatous polyposis, and a control group of 100 newborns and 100 aged individuals were included in this study. Out data indicate that FAP and HNPCC have relatively low frequency of 0.1% and 0,01); the concentration of P was for 79,85% higher in the pubertal persons compared with the persons over 65 years (p>0,001).
Conclusion: The obtained results suggest relationship between concentration of Ca and P with the age. The older persons have significant lower level of Ca and P compared with the young persons.
P24
CONJUGATION OF DIFFERENT SUBSTRATES WITH GLUTATHIONE CATALYSED BY GLUTATHIONE-S-TRANSFERASES T1-1
Yasemin AKSOY1, Anna-Karin LARSSON2, Bengt MANNERVIK2
1Hacettepe University, Faculty of Medicine, Department of Biochemistry, 06100, Ankara/Turkey,
2Uppsala University, Biomedical Center, Departmentn of Biochemistry Uppsala/Sweden
yaseminb@hacettepe.edu.tr
Glutathione S-transferase (GSTs; EC 2.5.1.18) is a detoxifying enzyme catalyzing the conjugation of glutathione with a variety of electrophilic substrates that can be either exogenous or endogenous. In mammals 8 different classes (alpha, kappa, mu, omega, pi, sigma, theta and zeta) of soluble GSTs have evolved with members that promote the detoxification of many structurally different electrophiles. The evolution of proteins for novel functions involves point mutations and recombinations of domains or structural segments.
Alkyl halides, epoxides and benzyl halides are substrates of GSTs. Substrates which were worked are industrial intermediates, laboratory reagents. It behaves as alkylating agents. Reports have shown them to cause the respiratory and dermal toxicity in animals and humans. It has also been reported to be carcinogenic in experimental models. Thus, the wide-spread use of these aliphatic epoxides, halides is of great concern in human health problem.
In our study, we purified GST T1-1 (h T1-1) from E.coli. GST activities towards 1,2 Epoxy-3-(4-nitrophenoxy)-propane(EPNP) and 4-nitrophenethyl bromide (NPB), styrene 7,8 oxide, acrylonitrile, benzyl bromide benzyl chloride, epichlorohydrin, glycidol were measured. Reactions of substrates with glutathione were measured by following the disappearance of glutathione. Glutathione was measured colorimetrically using Ellmans reagent (DTNB method). Results of DTNB method were compared with EPNP results. Activities were expressed as micromole of glutathione reacted per minute. The most active substrate which is epichlorohydrin with T1-1, substrate-saturation curve by varying its concentration (at constant GSH concentration) was prepared. Thus, Km and Vm values were determined.
P25
GENERATION OF IR IMAGES OF ALZHEIMER INFECTED TISSUE
Utku, H1, Miller Lisa M2
1. Institute of Nuclear Sciences, Hacettepe University, Beytepe, Ankara, Turkey
2. National Synchrotron Light Source, Brookhaven National Laboratory, New York, USA
Fluorescence-assisted synchrotron IR microspectroscopy permits us to identify target proteins with fluorochromes or fluorescent antibodies and simultaneously determine their secondary structure with IR micro-spectroscopy. Fluorescence microscopy may be used to identify amyloid plaques and tangles in the brain and other tissues of control. We have incorporated protein structure methods into Matlab to generate routines for image processing. Once the desired structural images are obtained, correlation analysis can be performed with the fluorescence microscopy images.
In order to generate IR images, the IR data was reduced to a relatively compact description through cluster analysis. The principal function of clustering is to display so that the influences or causes in arriving a pathogenic state might be predicted. Desired function of clustering is to reveal the protein structures in an infected tissue. Result of the cluster analysis can contribute directly to classification schemes. If the grouping suggested by the cluster analysis is to be adopted for operational use, then it may become the basis for classifying new observations. The developed software provides Linear Discriminant Analysis (LDA) option to perform classification based on the output of the cluster analysis.
P26
PossIble antIoxIdant effects of calcIum channel blocker In hypertensIon pregnant women
Didona Ungureanu1, Mihaela Maftei1, Cristiana Filip1, Nastasia Gheorghita1, Alexandrina Caba2
1.University of Medicine and Pharmacy Gr.T.Popa, Biochemistry Department, 700115, Iasi, Romania, didonaungureanu@yahoo.com
2.Cuza-VodaHospital, First Clinic, Iasi, Romania
Arterial hypertension in third trimester pregnant women is defined as values higher then 140/85 mmHg, one of the pathogenic mechanisms involved being intracellular calcium accumulation. Nifedipine- as a calcium channel blocker- in pregnant women hypertension will prevent calcium accumulation and thus, will restore blood pressure.
The study was carried out on 25 third trimester pregnant women, aged between 25 and 35 years old, diagnosed with pregnancy induced hypertension (PIH), and treated with nifedipine. The results were compared with a control group- 20 third trimester normal pregnant women. At both groups we dosaged enzymatic and nonenzymatic antioxidants parameters: superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione (GSH) and uric acid (UA). As marker of lipid peroxidation we used malondialdehyde (MDA). Under Nifedipine treatment antioxidant enzymes activities and GSH increased (p
