application of firefly luciferase assay for adenosine triphosphate (atp ...
These assays allowed measurement of major GPCR pathways including cAMP production, intracellular Ca2+ mobilizations, ERK/MAPK activ- ity, and ...
Improved Dual-Luciferase Reporter Assays for Nuclear ReceptorsA new in vivo assay system has been developed to study programmed frameshifting in the yeast Saccharomyces cerevisiae. Frameshift signals are inserted between ... chemiluminescence and bioluminescence measurements .pdfTD-20/20 luminometer. The following steps were used for luminescence mea- surements: 100 ?l of the fireffy luciferase reagent (LARII) was added to the test. Luciferase Reporter Assay System for Deciphering GPCR PathwaysThe firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for use in high-throughput screening (HTS) are ...
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